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978-3-8439-0079-9, Reihe Molekularbiologie
Jéssica Varela Villarreal
Molecular Biology Techniques Applied to Food Industry Water Surveillance
165 Seiten, Dissertation Karlsruher Institut für Technologie (2011), Softcover, A5
Culture - independent techniques were applied and optimized for the detection of patho- genic bacteria in drinking water at potentially critical control points along the production lines at a German dairy company and at a Spanish dry cured ham company. Denaturing gradient gel electrophoresis (DGGE) was used to describe bacterial population shifts indicating biological instability in drinking water and biofilm samples. Autochthonous bacteria were identified by sequencing the DNA bands excised from the DGGE gels. More specifically, polymerase chain reaction (PCR) and quantitative PCR (qPCR) were applied to detect a number of pathogenic bacteria, i.e. Listeria monocytogenes, Mycobacterium avium subsp. paratuberculosis, Campylobacter jejuni, Enterococcus spp., Salmonella spp., Escherichia coli, and Pseudomonas aeruginosa.
A specific strategy was established for the detection of possible water - derived critical control points at the food companies, where the technical detection requirements and the occurrence of unwanted polymerase inhibitions were contemplated.