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978-3-8439-1689-9, Reihe Biotechnologie
Oscar Platas Barradas
Process and Cultivation Strategies for the Human Industrial Cell Line AGE1.HN
130 Seiten, Dissertation Technische Universität Hamburg-Harburg (2014), Softcover, A5
This work focuses on the development of process and cultivation strategies for a systematic characterization and optimization of mammalian cell cultures, especially for the novel human industrial cell line AGE1.HN. This cell line was studied within the collaborative project SysLogics-Systems Biology of Cell Culture for Biologics- with the final goal of generating quantitative and systematic data on mammalian cell metabolism.
The strategies include a systematic comparison of process parameters in shake flasks and benchtop bioreactors with the main goal of guaranteeing consistency of the results within geometrically different cultivation systems.
Furthermore, this work aimed at developing a strategy for cell synchronization which should allow for a quantitative study of cell cycle dependent metabolic pathways. Here, physical methods like centrifugal elutriation were evaluated and experimentally tested for synchronization with further synchronous cell culture in bioreactors.
In the last part of this work a software tool for the evaluation of feeding strategies in Fed-batch culture was developed. This strategy aims to a reduction in the number of experiments during discretization of fed-batch strategies in mammalian cell cultures.
The strategies developed in this work establish a solid basis for systematic studies with mammalian production cell lines, by allowing for their characterization from an engineering point of view, and the implementation of results in systems biology research.